doi:10.16597/j.cnki.issn.1002-154x.2022.05.008
基质辅助激光解吸电离飞行时间质谱技术不同应用的简要概述
王聪1 王晓宇1 赵国强1 魏影1,2∗
(1. 佳木斯大学附属第一医院,黑龙江 佳木斯 154003; 2. 黑龙江省卫生健康管理服务评价中心,黑龙江 哈尔滨 150036)
a Brief Overview of the Different Applications of MALDI-TOF MS Technology
Wang Cong 1; Wang Xiaoyu 1; Zhao Guoqiang 1; Wei Ying 1,2∗;
(1. First Affiliated Hospital of Jiamusi University, Heilongjiang Jiamusi 154003; 2. Heilongjiang Province Health Management Service Evaluation Center, Heilongjiang Haerbin 150036)
摘要:
质谱技术的发展使人们能够通过直接分析不同的生物分子(如蛋白质、脂类和碳水化合物)来研究不同的生物系统,从而对生命科学领域产生重大影响。 近十年来,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)作为一种快速、可靠的微生物鉴定方法得到了广泛应用。 在本文中,我们简要概述了 MALDI-TOF 质谱技术基本原理及其在临床诊断、环境和食品监测等多个领域的微生物鉴定方面的不同应用。
关键词：MALDI-TOF 微生物检测 基本原理
Abstract：
The development of mass spectrometry technology enables people to study different biological systems by directly analyzing different biomolecules (such as proteins, lipids and carbohydrates), which has a significant impact on the field of life sciences. In the past ten years, matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS) had been widely used as a rapid and reliable method for microbial identification. In this article, we briefly outline the basic principles of MALDI-TOF mass spectrometry technology and its different applications in clinical diagnosis, environmental and food monitoring and other areas of microbial identification.
Keywords：MALDI-TOF; microbiological testing; basic principles;