doi:10.16597/j.cnki.issn.1002-154x.2023.06.006
水产品中地西泮残留量测定方法的建立及稳定性研究
张汉霞1 翁芳雁2 姚婉仪1 刘少彬1 胡浩光1 谢翠美1*
( 1． 东莞市动物疫病预防控制中心，广东 东莞 523000; 2． 广东省中鼎检测技术有限公司，广东 深圳 518000)
Establishment of a Method for Determination of Diazepam Ｒesidues in Aquatic Products and Study on Its Stability
Zhang Hanxia1 Weng Fangyan2 Yao Wanyi1 Liu Shaobin1 Hu Haoguang1 Xie Cuimei1*
( 1． Dongguan Animal Disease Prevention and Control Center，Guangdong Dongguan 523000; 2． Guangdong Zhongding Detection Technology Co．，Ltd．，Guangdong Shenzhen 518000)
摘要:
为了建立高效便捷的水产品中地西泮残留量的定量检测方法。作者通过实验研究得出: 使用氨水－乙腈溶液对样品进行提取，采用陶瓷均质子均质样品，吸附剂净化样品，氮吹浓缩样品，用液－质联用仪对地西泮进行检测，得到地西泮测试的曲线范围为 0.500 ～ 50.00 ng·mL－1 ，Ｒ= 0.99992; 样品出峰时间稳定且回收率范围为 90.8% ～ 110%。此方法具有省时、操作简便、检测回收率高、精密度好、出峰时间稳定的优势，可适合用于数量比较大的水产品样品中地西泮的检测。
关键词：地西泮检测 水产品 液－质联用仪 方法的建立 稳定性研究；
Abstract：
In order to establish an efficient and convenient quantitative method for the determination of diazepam residues in aquatic products． Based on the experimental study，the following results were obtained: extraction of samples with ammonian-acetonitrile solution， ceramic proton homogenize sample， adsorbent purification sample， nitrogen blowing concentration sample， detection of diazepam with liquid chromatograph-mass spectrometer combination instrument，the test curve range of diazepam was obtained as 0.500 ～ 50.00 ng·mL－1 ，R= 0.99992; The peak time of the samples was stable and the recovery range was 90.8% ～ 110%． This method has the advantages of time-saving，simple operation，high detection recovery，good precision and stable peak time， and is suitable for the detection of diazepam in aquatic product samples with a relatively large number of detection．
Keywords：detection of diazepam; aquatic products; liquid chromagraph-mass spectrometer; method establishment; study on stability;