配体交换反应分光光度法测定依地酸钙钠注射液含量

DOI: 10.16597/j.cnki.issn.1002-154x.2024.06.006

作者：张淳、朱嘉华、高雪倩、杨心语、马卫兴

单位：1. 江苏海洋大学药学院，连云港 222005；2. 江苏海洋大学生物医药产业学院，连云港 222005

摘要：pH为5.00时铜离子与锌试剂及新洁尔灭发生反应形成绿色的三元配合物，其最大吸收波长位于610 nm。加入依地酸钙钠[EDTACa]Na2可发生配体交换反应，形成依地酸铜钙配合物[EDTACu]Ca，并使原绿色三元配合物分解而导致体系褪色。通过条件优化建立了配体交换反应分光光度测定依地酸钙钠的新方法，其工作曲线的线性回归方程为A=0.049C+0.0125，相关系数R=0.9996，依地酸钙钠的含量在1~12 mg·L-1时符合Beer定律呈现线性关系，经实验测试，可成功应用于依地酸钙钠注射液中依地酸钙钠含量的测定，所拟方法简单、快速。

关键词：铜、锌试剂、依地酸钙钠、配体交换反应、分光光度法

Authors: ZHANG Chun, ZHU Jiahua, GAO Xueqian, YANG Xinyu, MA Weixing

Affiliation: 1. School of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China; 2. School of Biomedical Industry, Jiangsu Ocean University, Lianyungang 222005, China

Abstract: The green ternary complexes were formed by the reaction of copper ion, zincon, and bromogeramine in the pH 5.00 buffer solution, with the maximum absorption wavelength of the complexes at 610 nm. The ligand exchange reaction led to the fading reaction on decomposition of the green ternary complexes and formation of the calcium copper edetate complexes [EDTACu]Ca by addition of calcium disodium edetate [EDTACa]Na2. The new method was proposed for assay determination of calcium disodium edetate with the ligand exchange reaction spectrophotometric method under optimization, whereas the linear range obeyed the Beer's law at the calcium disodium edetate concentration ranges of 1-12 mg·L-1 with the linear regression equation of the working curve A=0.049C+0.0125 and the correlation coefficient R=0.9996. The proposed method was simple and fast to successful assay determination of calcium disodium edetate injection.

Keywords: copper, zincon, calcium disodium edetate, ligand exchange reaction, spectrophotometric method